| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_76fe5493135e982263e13db45ae2ec69
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_63a820378f7fb6b5e85aa10d5a631ef6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_35534d598254337e8326ba65aac83150
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_385ebd16835d967440a4f58f67fac36b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ea7da26cb077e35d67c7703ba5e49d7d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_584190b1b081605cde60dc2c299c4317 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-831
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10S530-83 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P7-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-755 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P7-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-755 |
| filingDate |
1995-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d1cbd92a916215475da50eb3ee289c5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9953dcd2570c358f3887a5083ea390d3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf4362e0a9725904573b94a32842117b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e481417014a70989be94c8943d20c1b3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_acbf36afbbbd158718219ef3526b60f6 |
| publicationDate |
1996-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-9615150-A1 |
| titleOfInvention |
Process for purifying factor viii |
| abstract |
Proteases generally tend to reduce the activity of coagulation factor VIII by degrading the molecule. The present invention relates to a process for reducing the detrimental influence of metal-dependent proteases upon recombinant factor VIII molecules, by adding an inhibitor of metal-dependent proteases to a solution after harvest. The solution is suitably the harvest solution or any aqueous solution being fed to or leaving the primary isolation, being the initial step of the purifying sequence. The inhibitor is selected from: i) complexing agents with a stronger affinity for the metal ion of the protease than for the ion or ions stabilizing the factor VIII molecule and ii) compounds structurally related to the natural substrate of the protease and containing an electronegative moiety. The presence of an inhibitor, preferably a complexing agent, after harvest allows for a prolonged harvest period and considerably higher yield with essentially retained factor VIII activity. The invention further relates to an aqueous solution containing recombinant factor VIII which has been purified according to the present process and use of such an aqueous solution, for the manufacture of a medicament for administration to a patient having the symptoms of hemophilia. Also, the invention relates to a method for treatment of hemophilia by administration of a therapeutically effective amount of recombinant factor VIII which has been purified according to the present process. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008135498-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10512674-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-11020459-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8765665-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8372800-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9669076-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8969041-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9352027-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2008135498-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011027152-A1 |
| priorityDate |
1994-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |