Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f77a2093324f9c8ae0fbe64e4de82c39 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-144444 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-805 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-00 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-48 |
filingDate |
1995-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_33cd090badda0b88dd97a9c1c7c46921 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4b8e693b1e0963648f6b9b548c241c4f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a45458b86537f608be6b35e583b9dd4 |
publicationDate |
1995-12-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-9533072-A1 |
titleOfInvention |
Assay for enzyme activity using a microfluorometric assay |
abstract |
The present invention is a method for assaying enzyme activity in a red blood sample. The method comprises theses steps: (a) placing the following in a sample well; 1) a red blood sample containing an enzyme, 2) a substrate or substrates for the enzyme, 3) water, and 4) a buffer; (b) incubating the contents of the sample well for sufficient time and at sufficient temperature to allow for the formation of a fluorescent enzyme product should the enzyme be present in said red blood sample; (c) precipitating the hemoglobin; and (d) measuring the fluorescence of any fluorescent enzyme product formed in the sample well, directly from that sample well. The method of the invention may be used for assaying the activity of an enzyme, such as galactose-1-phosphate uridyl transferase (GALT) or biotinidase, in a red blood sample. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105372218-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105372219-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-105334203-A |
priorityDate |
1994-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |