| abstract |
Electrophoretic media and devices comprising said media are provided, wherein said media is capable of being reversibly converted in response to an applied stimulus from a first state of low viscosity to a second state of high viscosity, which is continuous and electrophoretically sievable. The subject media comprise a continuous fluid phase, a polymeric composition, such as discrete microgel particles that can be swollen and collapsed in response to the applied stimulus, and optionally a binder. The subject media find use in electrophoretic devices comprising a gel holder, such as slab gel devices, column and capillary devices. Among other advantages, the subject media provide for ease of preparation, superior gel formation and improved resolution of electrophoretically separated components. |