abstract |
Defective poxviruses that lack a function imparted by an essential region of its parental poxvirus can be employed for protein production and vaccination, among other uses. A polynucleotide encoding the protein to be produced by the recombinant virus is inserted into the defective poxvirus and placed under transcriptional control of a promoter. The defective poxvirus is viable when the lost function of the essential region is complemented by a host cell, transgenic animal or helper virus. |