abstract |
A process of separating milk clotting enzymes in extracts of animal stomach tissue which comprises contacting a partially purified extract with an ion exchange resin under conditions where chymosin is bound to the resin and recovering the chymosin, the process optionally comprising the further step of recovering pepsin, from a pepsin-rich extract fraction using an ion exchange resin, and a liquid or powdered rennet composition containing at least 90 % of its milk clotting activity as chymosin activity, the composition comprising a chymosin stabilizing agent which is typically selected from a protein, a peptide, an amino acid or ascorbic acid. |