http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9511980-A2
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_61f88efcda59d7062a4252ecf6886f58 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-24222 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-18 |
filingDate | 1994-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3632d367ca7205e83a19cfb9d487dbc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_31d4679f2d93e621ba5fe690559ad540 |
publicationDate | 1995-05-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-9511980-A2 |
titleOfInvention | PLASMIDS FOR EFFICIENT EXPRESSION OF SYNTHETIC GENES IN $i(E. COLI) |
abstract | The invention provides a vector for efficient expression of a synthetically produced protein coding nucleotide sequence comprising a vector comprising in sequential order 5' to 3': a) a Shine-Dalgarno nucleotide sequence having a unique restriction endonuclease site and b) a synthetically produced protein coding nucleotide sequence having i) a translation start codon; ii) a sequence about equidistant 3' of the translation start codon as the Shine-Dalgarno sequence is 5', which selectively hybridizes with the Shine-Dalgarno sequence to form a hairpin loop wherein the start codon is exposed in the loop such that translation is efficiently initiated; and iii) an adenosine-containing region of a length sufficient to substantially prevent secondary structure of the vector in the region immediately downstream of the hairpin loop. In particular is provided a vector providing a synthetic gene encoding the hepatitis C virus nucleocapsid protein that can be efficiently expressed in prokaryotic cells. |
priorityDate | 1993-10-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 431.