| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e66508ec06bf48ba74c9d4a6b5109474
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_56666880a5e2b7703e98a4f9b2d9daf6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a40da629ea3695a5c8e2a2e471f18045
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0dc6695e922848ef4ae13c83fcc55755
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e93889a0eac2e989719e50cc9cbb2257 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-14143 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-866
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 |
| filingDate |
1993-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ff315294b82d970170765f2d7679eff
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_39b97427efc55b35fc085665dd23b19c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_704cefd5aff8afe9e5f3eb12f02156e7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_586f1a15ce32b7d96672774b35af8e4a |
| publicationDate |
1995-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-9509923-A1 |
| titleOfInvention |
Method of producing recombinant eukaryotic viruses in bacteria |
| abstract |
A method for producing infectious recombinant baculoviruses in bacteria is described. A novel baculovirus shuttle vector (bacmid) was constructed that contains a low-copy-number bacterial replicon, a selectable drug resistance marker, and a preferred attachment site for a site-specific bacterial transposon, inserted into a nonessential locus of the baculovirus genome. This shuttle vector can replicate in E. coli as a plasmid and is stably inherited and structurally stable after many generations of growth. Bacmid DNA isolated from E. coli is infectious when introduced into susceptible lepidopteran insect cells. DNA segments containing a viral promoter driving expression of a foreign gene in insect cells that are flanked by the left and right ends of the site-specific transposon can transpose to the attachment site in the bacmid propagated in E. coli when transposition functions are provided in trans by a helper plasmid. The foreign gene is expressed when the resulting composite bacmid is introduced into insect cells. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-8252278-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7135337-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9844141-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023028440-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1144666-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111808884-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7413732-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9844141-A2 |
| priorityDate |
1993-10-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |