Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f4e2aab96eefa9175ac33e02f4c0cfa3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a32fa7450dcdaad70c4072b4fb63c441 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_da29df70e9d467fa1f840206d2e00304 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ee1c19da359446fb5c4f0458a57b783d |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-5412 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-815 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4721 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-765 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-765 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 |
filingDate |
1994-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2475f6a3779e273cda52bc7e5f8af577 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b736a171d2b34a5a5fc02ede030e6ab http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c6c17861cd6a2bfb82e8f05cbd100a0 |
publicationDate |
1995-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-9509914-A1 |
titleOfInvention |
Multicloning vector, expression vector, and production of foreign protein with expression vector |
abstract |
An expression vector capable of expressing in a fission yeast Schizosaccharomyces pombe; a multicloning vector for constructing the expression vector; a method of constructing the expression vector; a transformant holding the expression vector; and a process for producing a foreign protein by using the transformant. The multicloning vector has a sequence of -ACATGT- starting with the 5' side at the tip of the multicloning site as the restriction enzyme recognition site for introducing a structural gene of a foreign protein. An expression vector is constructed by introducing a structural gene of a foreign protein by using the above recognition site in such a manner that the tip of the structural gene will have a sequence of -ACATGN- (N being an arbitrary base) starting with the 5' side and that the ATG region of the sequence will function as a translation initiation site of the structural gene. Since it is possible to use an arbitrary base as the N in the sequence of ATGN of the expression vector, it becomes possible to introduce a structural gene of any foreign protein and to express the structural gene to a high level. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9284561-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2014030644-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2011021629-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2015076393-A1 |
priorityDate |
1993-10-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |