| abstract |
The present invention provides novel DNA antibody and DNA antibody fragment conjugates. In particular, the invention comprises bis-conjugates of antibodies or antibody fragments, especially Fab' antibody fragments, cross-linked by an end-derivatized double stranded DNA. The conjugates can comprise homo- or hetero-, bi-, tri-, or tetra-specific antibodies and the DNA can contain one or more restriction enzyme sites. The invention provides general techniques for preparing the double stranded DNA-antibody and double stranded DNA-antibody fragment conjugates and for using them to measure the distance and study the relationship between cell surface proteins. The invention also provides an immunoassay using part of the technique for preparing the conjugates and a method for using the DNA-conjugates in immuno-PCR assays to coincidentally detect more than one antigen in a sample. |