| abstract |
The invention provides a means for attaching a label, support or bioactive agent to the protein at a truncating site within the protein. More specifically the invention is directed to a method for the attachment of an amino acid, amino acid derivative, peptide or polypeptide, nucleophile to a protein core obtained from a protein of the core and a leaving group. In one embodiment, an endopeptidase enzyme is utilized to attach a labeled nucleophile to a protein core such as an antibody. In other embodiments, the invention is directed to a method for the attachment of a protein core to an immobilization support and to a method for the attachment of a bioactive agent to a protein core. |