| abstract |
A method for detecting the presence of any of the materials C1, C2, enzyme E1 (having the substrates C1 and S1) and enzyme E2 (having the substrates C2 and S2) that undergo the following reactions (I) and (II). This method comprises adding the other materials sufficient for the reactions to proceed, and observing the loss of S1 or S2 or the formation of P. The formation of P increases exponentially, and that can be used to give a colour reaction quickly and simply, with a suitable P-linked reaction, in the presence of microorganisms (C1 being ATP). |