Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ba485f05b809aa40b1283026b93bda47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f055bf58f0b16d53127fb8e2ab912b05 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ce5c762d862c17af043a115954537277 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-21 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
filingDate |
1994-02-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f2a7921cb2f23abb4d0192c66061c8a2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_959b3e639df7a57af287f901d64f5372 |
publicationDate |
1994-09-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-9419472-A2 |
titleOfInvention |
Bi-functional expression system |
abstract |
A novel expression system is provided comprising a DNA sequence containing transcriptional and translational signals that promote the over production of recombinant proteins both in bacterial hosts (e.g., Escherichia coli) and yeasts (e.g., Saccharomyces cerevisiae). The design of the expression system lends itself to a unique strategy which allows heterologous genes to be directly cloned at a position relative to the transcription/translation signals which is optimal for expression. Particularly provided are expression cassettes comprising a sequence of the invention combined with a purpose built series of plasmids wherein the utility and efficiency of the resultant expression vectors can be demonstrated to over produce protein, particularly phenylalanine ammonia lyase (herein abbreviated to PAL), in E. coli and S. cerevisiae to levels hitherto unattainable. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7494801-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9904020-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1574565-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1574565-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6303345-B1 |
priorityDate |
1993-02-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |