| abstract |
A cloning and/or sequencing vector (1) comprises, incorporated in an autonomous replication vector (2), at least one nucleotide promoter sequence (3) and at least one nucleotide sequence (4) coding for a fusion protein active as a poison. Said nucleotide sequence (4) is obtained by fusion of a coding nucleotide sequence (5), comprising several unique cloning sites and a nucleotide sequence (6) coding for a poison protein. The vector host cell of the invention is also disclosed. |