| abstract |
Granule proteases having DNA fragmenting activity and useful in targeting tumor cells to cause nuclear collapse and apoptosis in the tumor cells have been isolated and purified. In particular, these proteases, termed fragmentin 1, fragmentin 2 and fragmentin 3, have non-reduced apparent molecular weights of 47 kD, 31 kD and 27 kD respectively, have been isolated from a rat natural killer tumor cell line by solubilization of rat RNK cell granules and purification of the granular proteases. |