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classificationIPCInventive http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543
filingDate 1991-11-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d160d8b681974203e92a957fdd4d3d0
publicationDate 1993-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-9310453-A1
titleOfInvention Immunological detection method and device
abstract A method for quickly, quantitatively and qualitatively determining the presence of a reactive ligand in a fluid, wherein one or more specifically labelled reagents (in particular antibodies or antigens) are recognized and trapped by one or more unlabelled reagents (in particular specially prepared immunoglobulins) which are immobilized on a support of any given kind, when said labelled reagents are not complexed with their ligand; and conversely, the labelled reagents are neither recognized nor trapped by the unlabelled reagent(s) when said labelled reagents are complexed with their ligand. The method may be used in ELISA plates, where an antibody specific for a desired ligand is immobilized on the walls. When a ligand is present in the sample, its Fab sites are masked and go unrecognized by a labelled anti-Fab immunoglobulin, but when the ligand is absent, the labelled anti-Fab immunoglobulin is bound to the immobilized antibodies, so that the signal is inversely proportional to the ligand level, and the problem of background noise is completely eliminated.
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priorityDate 1990-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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