Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_058e0be30e00d53858c67167df60aed5 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56961 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5308 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-573 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
filingDate |
1992-03-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_502064f627636df2300efa55e87bc234 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_defed970e35890ae25142bed297cef92 |
publicationDate |
1992-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
WO-9217786-A1 |
titleOfInvention |
Assay device and method of detecting chitin |
abstract |
A method for detecting chitin-containing organisms such as fungi, yeast and insects in a sample attached to a solid phase, by detecting the presence of chitin by using enzymes which specifically bind chitin is disclosed. The method is preferably carried out by passing a sample which contains chitin through a membrane in order to capture and adhere the chitinous material on the membrane. A chitin-binding enzyme such as chitinase or lysozyme is added under conditions which allow it to bind to the chitin on the membrane. A signal generating compound is either directly conjugated to the chitinase (or lysozyme) or indirectly conjugated to either an anti-chitinase (or lysozyme) antibody or to an anti IgG antibody (which binds to the anti-chitinase antibody) to detect the chitin-chitinase complex. Reagents for the signal generating compound then are added to produce a detection signal which indicates the presence of the chitin. Kits which contain the necessary materials for carrying out the methods of the invention are also disclosed as are novel conjugates. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2012025619-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5914239-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5935804-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9842864-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6440388-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5518890-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005005955-A2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6159719-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6372446-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9802742-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9516879-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6090573-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9833934-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6184027-B1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5997846-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9380781-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9933425-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6121420-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10677796-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10094829-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2005005955-A3 |
priorityDate |
1991-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |