http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9217504-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d21cbff64022f95c237c0bd3ec8656ca |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-52 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-52 |
| filingDate | 1992-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2e59c62ee59f387a96ad174f607b6dc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1ba71f24f5fb311c6b3d91d4afeaa0c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77c42d00561e77f1ad3b39726e3d48cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3b9d5735486cf7a550523f81d567d9a0 |
| publicationDate | 1992-10-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-9217504-A1 |
| titleOfInvention | Macrophage cytotoxin compositions and methods of preparation |
| abstract | This invention provides a cytotoxin composition that inhibits mitochondrial respiration and causes cytostasis in cells, independent of nitric oxide. The invention comprises a conditioned supernatant derived from macrophage cell line (EA13.5) that inhibits mitochondrial respiration and causes cytostasis in cells. More particularly, the invention comprises a macrophage cytotoxin obtained from conditioned supernatant collected from cultured EA13.5 cell. This macrophage cytotoxin (1) inhibits mitochondrial respiration, (2) causes cytostasis independent of L-arginine derived nitric oxide, (3) is a weakly acidic (pH of about 7.5 to 8.0) glycoprotein (as determined by binding to lentil lectin sepharose). The invention also discloses a method for producing an L-arginine-derived nitric oxide-independent macrophage cytotoxin by culturing EA13.5 cells, adding IFN-$g(g) and lipopolysaccharide to trigger secretion of the macrophage cytotoxin into the conditioned supernatant, collecting the conditioned supernatant, and isolating the macrophage cytotoxin from the conditioned supernatant. Further disclosed by this invention is a method for producing a nitric oxide independent macrophage cytotoxin, that inhibits mitochondrial respiration independent of L-arginine, from cultured rodent macrophages. |
| priorityDate | 1991-04-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 302.