| abstract |
DNA molecules encoding human galanin are provided. The molecules are used within methods for detecting galanin antagonists through the use of recombinant DNA techniques. Briefly, DNA sequences encoding galanin analogs are generated and expressed in suitable host cells. The analogs are exposed to a galanin receptor coupled to a response pathway in the presence of native galanin. A reduction in the inhibition of the response pathway resulting from the binding of the galanin analog to the galanin receptor, relative to the inhibition of the response pathway by native galanin alone indicates the presence of a galanin antagonist. Galanin antagonists identified and isolated through these methods are also provided. |