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filingDate 1991-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1992-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-9208976-A1
titleOfInvention Method for determining lipid bound sialic acid in plasma
abstract The amount of lipid bound sialic acid in a blood plasma or serum sample may be determined by an improved method which may be automated involving the following steps to be performed simultaneously on the sample and a known standard: diluting with buffer; mixing the diluted sample; adding a mixture of a chlorinated lower alkyl alcohol; mixing, diluting with buffer and then treating by mixing further and centrifuging to yield a substantially clear upper phase; recovering the upper phase and adding to it a protein precipitating agent, mixing the resulting admixture; recovering the resulting precipitate, suspending the precipitate in a hydrolysis agent and determining the amount of lipid bound sialic acid present by comparing the optical density of the sample to the optical density of the standard. An improved standard for this method is provided as well.
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