| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_03721e344a72026d125291e6c56c1be6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_33b011e77b33662b8bf1f2ff2de7b661 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-701
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-683 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
1991-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e193aac6e1fd858e8af2d4c0e6c9a19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44f9e3aba3eddf67153e34ebdc4341dd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3448c0be93eba935215d8298f3a5b30a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_95cf325e2e645fb6c0ec1c741f8bb9f6 |
| publicationDate |
1992-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-9207958-A1 |
| titleOfInvention |
Test for virulent revertants in attenuated live vaccines |
| abstract |
The present invention concerns a method of evaluating the safety of live attenuated vaccines which is based on detection and measurement of the incidence of genetic changes associated with reversion to virulence in vaccine microorganisms. The method based on PCR and restriction enzyme analysis was developed and used for determination of the proportion of mutants contributing to neurovirulence of type 3 live oral poliovirus vaccine. The correlation between the neurovirulence of OPV lots revealed by the monkey test and the abundance of mutant virus containing cytidine in the positon 472 was discovered. The amount of these mutants increases upon passages of the virus in cell cultures at a rate dependent on the cell type, cultivation conditions and the seed virus stock. The present invention can be applied for the safety test of lots of live vaccines and in-process control of vaccine manufacturing as well as the approach for the optimization of conditions for manufacturing of safe vaccine and selection of the seed virus and cell substrate. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5986171-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9514107-A1 |
| priorityDate |
1990-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |