| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_45904fc578f68d9d6facd26f2047f7b3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_22853c8669cb52e7d6fea1eea6467958 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate |
1991-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_230bf0ff9eae4708d8bf7b286cca38d7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d68da7bebf73941af9f767f2799b871d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a95d459625b0498d16d7cba5cc339ce1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_717f0bbfd7150e69d7b06f618691df2a |
| publicationDate |
1992-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-9206189-A1 |
| titleOfInvention |
Direct cloning of pcr amplified nucleic acids |
| abstract |
Methods are described for producing recombinant DNA molecules from suitable host vectors and nucleic acids subjected to 3'-terminal transferase activity. In one embodiment, the method takes advantage of the single 3'-deoxy-adenosine monophosphate (dAMP) residues attached to the 3' termini of PCR generated nucleic acids. Vectors are prepared with recognition sequences that afford single 3'-terminal deoxy-thymidine monophosphate (dTMP) residues upon reaction with a suitable restriction enzyme. Thus, PCR generated copies of genes can be directly cloned into the vectors without need for preparing primers having suitable restriction sites therein. The invention also contemplates associated plasmid vectors and kits for implementing the methods. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-1775345-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5426039-A
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-100453279-B1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03091440-A1 |
| priorityDate |
1990-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |