| abstract |
The present invention relates to recombinant β-1,3-glucanase essentially free of proteases. The enzyme is obtained through the use of a recombinant DNA expression vector which comprises a DNA sequence encoding the β-1,3-glucanase gene or mutants and variants thereof placed under the control of an exogenous expression promoter, preferably a bacterial promoter. Also, the β-1,3-glucanase gene may include sequences flanking the open reading frame of the native β-1,3-glucanase gene. The present invention also relates to a microorganism transformed with a recombinant DNA expression vector comprising the β-1,3-glucanase gene or mutants and variants thereof under the control of an exogenous expression promoter. |