| abstract |
In the field of bone marrow transplantation and adoptive immunotherapy, methods for in vitro culturing therapeutic quantities of bone marrow cells are provided. Bone marrow cells, which may be autologous or obtained from a donor, are cultured in a hollow fiber bioreactor culture system. The bone marrow cells may be pre-treated with a chemotherapeutic or other agent, or cultured in the presence of at least one chemotherapeutic or other agent or growth promoting substance. Heterologous DNA may be introduced into the bone marrow cells during culture. Bone marrow cells cultured according to the methods of the invention may be used in adoptive immunotherapy procedures to treat cancer, genetic disorders and AIDS. |