abstract |
A silver enhanced gold-labelled immunogold assay (SEGLISA) capable of determining levels of target analytes such as antigens, antibodies and drugs in physiological fluids, including whole blood samples, is disclosed. The assay is particularly suited for the determination of the presence and/or amount of HIV and Rubella antibodies and provides a permanent result of less equivocal nature than prior ELISA assays. Assay of samples provided by spotting whole blood onto blotting paper by the technique opens up the possibility of submitting samples by post. |