| abstract |
A process is described for the immobilization of proteins, peptides, ligands, coenzymes, redox mediators, etc., on a substrate with amino, mercapto or hydroxy groups. The substrate is reacted with a compound of general formula (I) or (IA) in which X is a halogen and R1 and R2, which may be the same of different, are X, R, COR, COOR (R being a C1-C8 alkyl group), COOH, CNS, N3 or CN, and optionally dried. The peptides, proteins, coenzymes, etc., to be immobilized are then reacted with the free halogen in the ortho or para position relative to the C=0 group in the compound of general formula (I) or (IA). |