abstract |
A recombinant thermophilic NAD-dependent dehydrogenase having a hydrophobic amino acid at position 104 and/or 102. The enzymes are effective in catalysing the dehydrogenation of homologues of pyruvic acid of formula CnH2n+1COCOOH, wherein n is > 1, to homologues of lactic acid of formula CHnH2n+1CHOHCOOH, wherein n is > 1. |