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filingDate 1990-01-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_87f0b407589c862bff094506536afa44
publicationDate 1990-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-9008398-A1
titleOfInvention A method of preparing samples for mass analysis by desorption from a frozen solution
abstract A method is disclosed for the processing and preparation of a sample (7) of one or more nonvolatile or thermally labile molecules for mass spectral analysis which comprises providing a solution of such molecules dissolved in a solvent, freezing the solution of dissolved molecules to form a frozen solution (8), and exposing the frozen solution to a source of energy (30), to desorb the molecules to be analyzed from the surface of the frozen solution. The desorbed molecules may be directly ionized during desorption or may be photoionized after desorption (40), if necessary, and then conventionally analyzed in a mass spectrometer (60). The method mitigates or minimizes possible fragmentation or clustering of such large molecules during desorption from the frozen sample as well as providing a more reliable and uniform desorption. If desired the desorbed molecules may be subjected to a separate and controlled fragmentation step prior to entering the final mass spectral analysis zone. The method may be interfaced with liquid chromatography equipment.
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