http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9007860-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bd8a00f7d73b12e7303e0c4620587a49 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2531-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0601 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-07 |
| filingDate | 1989-12-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03f24691904bad2a3d4b857f38eeb7e2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2a272bbfd11dcae1b4b7472bcd11f775 |
| publicationDate | 1990-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-9007860-A1 |
| titleOfInvention | Production of horseshoe crab amebocytes in vitro |
| abstract | A method for producing horseshoe crab amebocytes $i(in vitro) by isolating amebocyte producing tissue from the gill flaps of horseshoe crabs, especially $i(Limulus polyphemus). In contrast to all previous attempts to isolate and culture amebocytes from $i(Limulus) for the production of pyrogen-sensitive lysate, an effective method and means have been developed utilizing the discovery that the gill flaps are the source of the cells which differentiate into competent amebocytes. Once isolated, the tissue can be cultured long term $i(in vitro) on an artificial surface or as part of the gill flap leaflets, opened along one edge to allow access of the media to the developing amebocytes. Amebocytes are removed from the gill flaps by pulsing with $i(Limulus) serum, copper sulfate, detergent, or combinations thereof. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-7479543-B2 |
| priorityDate | 1988-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.