| abstract |
Disclosed are (a) aqueous indicator solutions useful for detection and quantitation of peroxidatic activity, containing a hydroperoxide and a chromogenic organic electron donor of the benzidine family, which possess the novel property of developing negligible color and maintaining approximately full sensitivity during storage at 0-40°C on a time scale minimally of six months; (b) indicator solutions for peroxidatic activity wherein the chromogen is o-phenylenediamine and storage stability is minimally for one to two days at room temperature or two weeks at 5°C; (c) certain chelators which promote these storage stability goals for o-phenylenediamine and for the benzidine compounds; and (d) processes for preparing such indicator solutions, comprising measures for minimizing contamination by oxidatively active transition metals. The resulting solutions increase the simplicity and reliability of a wide array of chemical, biological, and clinical tests, where either the analyte is a peroxidatic catalyst or the analyte is detected through the specific binding of another molecule, linked directly or indirectly to a peroxidatic catalyst. |