| abstract |
A procedure for the routine preparation in closed system and storage in a medium made of natural colloid (plasma) and crystalloid solution of PC deprived of leukocytes, which is based on a quadruple blood bag (PB) containing CPD anticoagulant, an empty satellite bag (S1), a satellite bag (S2) containing a synthetic crystalloid solution, a satellite bag with SAGM (or similar) additive solution for red blood cells (RBC), consisting in collecting the blood in the primary bag (PB), in centrifuging the blood bag at high speed, in expressing the supernatant plasma into bag (S1), in collecting 15-20 mL of the buffy-coat (BC) into bag (S2), in adding the SAGM solution of bag (S3) into bag (PB) containing the RBC concentrate, in disconnecting bags (PB) and (S1) from bags (S2) and (S3), in centrifuging bag (S2) at soft spin and in transferring the supernatant platelet concentrate (PLP-PC) formed after this centrifugation into the empty bag (S3), through a cotton wool filter (F) inserted along the tube line so as to remove residual leukocytes which contaminate the PC. |