| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ec7e91a4bbb290ad1ff1500777c66d67 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-141111 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6824
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6842
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-128
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-122 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-12 |
| filingDate |
1989-03-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75ba94202d439e34f33dc7a1cda71b48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4cfc303ad6339a1fdd6974445554ecc2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba4cbae55c9ea26e64b5dcdc8145a902 |
| publicationDate |
1989-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-8908830-A1 |
| titleOfInvention |
Thioacylation peptide sequencing with alcoholysis of thiazolinones |
| abstract |
A novel sequencing method which exploits the thioacylation degradation of polypeptides and proteins is disclosed. The process involves reaction of the N-terminal amino acid of a polypeptide with an excess of a thioacylating reagent. After sufficient time to insure quantitative coupling and removal of excess reagent, the N-thioacyl polypetide is subjected to cleavage by acid which affords a 2-substituted-5(4H)-thiazolinone of the N-terminal amino acid. Subsequent addition of a large excess of an aliphatic primary or secondary alcohol, either directly to the cleavage acid or after its removal, yields the corresponding N-thioacyl amino acid ester, a stable compound suitable for chromatographic separation and subsequent detection by contemporary methods of high pressure liquid chromatography. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9214702-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6818651-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2013144228-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5534440-A |
| priorityDate |
1988-03-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |