http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-8901487-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ec7e91a4bbb290ad1ff1500777c66d67 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-685 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-122 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K5-081 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-685 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K5-083 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-12 |
| filingDate | 1988-08-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75ba94202d439e34f33dc7a1cda71b48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba4cbae55c9ea26e64b5dcdc8145a902 |
| publicationDate | 1989-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-8901487-A1 |
| titleOfInvention | Removal of n-terminal acylated amino acid residues from polypeptides |
| abstract | The present invention discloses a two-step process by which an N-terminal-alpha-acylated amino acid residue may be removed from a polypeptide with the concomitant release of the residual polypeptide, wherein the N-terminal amino acid residue is unmodified and suitable for chemical degradation for the purpose of determining its primary structure. In the first step of the process a polypeptide comprised of n+1 amino acid residues, and containing an N-terminal-alpha-acylated amino acid residue, is reacted with a dithiophosphetane reagent, in tetrahydrofuran, dioxane or acetonitrile, at room temperature or at reduced temperature. Removal of the byproducts of the reaction yields a polypeptide of n+1 amino acid residues wherein the N-terminal-alpha-acylated amino acid residue has been converted into the corresponding N-terminal-alpha-thioacylated amino acid residue. In the second step the thioacylated polypeptide is reacted with an anhydrous organic acid, at room temperature, resulting in the removal of the N-terminal amino acid residue as the 2-methylthiazol-5(4H)-one or 2H-thiazol-5(4H)-one, and the concomitant release of the residual polypeptide comprised of n amino acid residues wherein the new N-terminal amino acid residue is unmodified. |
| priorityDate | 1987-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 103.