http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-8900887-A1

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filingDate 1988-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_485f6d31c63e3967c22535a83ff62dfd
publicationDate 1989-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-8900887-A1
titleOfInvention Apparatus and method for immunohistochemical staining
abstract A device and method for automating immunohistochemical staining of biological material on glass slides is presented. The slides (21) are placed on the base (1) with the tissue section facing upwards. A ''chamber'' consisting of a rectangular plastic box (4) with a hinged overhead door (86) is clamped directly over the tissue section. A rubber gasket (7) seals the chamber-slide interface. Reagents are dropped on the tissue section by means of the hinged overhead plastic door. When the door is closed, the chamber is sufficiently sealed so as to prevent evaporation. Washing of the tissue section is accomplished by the addition and aspiration of buffer through ports (14, 10) in the chamber. The ports are connected via flexible tubing (14a, 10a) to a manifold (11, 15). Because the wash steps are performed in tandem and do not require manual manipulation of slides, a significant time saving is realized. A mesh (30) may be placed between reagents and the surface of the slide (21) to spread a reagent over the surface of the slide.
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priorityDate 1987-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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Total number of triples: 31.