| abstract |
A reagent for classifying leukocytes, which enables classification and counting of leukocytes by lysing erythrocytes and acting on leukocytes and which contains at least one surfactant selected from the group consisting of (a) a first group of surfactants of polyoxyethylene anionic surfactants represented by the general formula: R1-R2-(CH2CH2O)n-X (wherein R1 represents alkyl, alkenyl or alkynyl having 10 to 22 carbon atoms, R2 represents O, (alpha) or COO, represents an integer of 8 to 30, and X represents SO3Na, COONa, OSO3Na or ONa) and (b) a second group of polyoxyethylene nonionic surfactants represented by the general formula: R1-R2-(CH2CH2O)n-H (wherein R1 represents alkyl, alkenyl or alkynyl having 10 to 22 carbon atoms, R2 represents O, (alpha) or COO, and n represents an integer of 8 to 30). A method of classifying leukocytes into three to five groupes of lymphocytes, monocytes, and granulocytes according to particle analysis methods, such as the RF method or the DC method, using said reagent is also disclosed. The reagent may contain an osmotic pressure increasing agent and a solubilizing agent. This method enables classification of leukocytes into 3 to 5 types and differentiation of normal cells from abnormal cells. |