| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ed28726de9f916f355f8adc331e736b8 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-91 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K45-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K9-127 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-195
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K9-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P1-00 |
| filingDate |
1987-04-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea567a3e163479612ffb8e07a8252392
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b8f8364da3a188a9ec7fce33e5ec208c |
| publicationDate |
1987-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-8706589-A1 |
| titleOfInvention |
Method for preparing contact-inhibitory factor |
| abstract |
A method for preparing a biologically active factor that is capable of restoring contact-inhibition of growth in malignant cell type. The method comprises loading a mixture comprising culture medium conditioned by growth of a contact-inhibited cell culture and an ionic buffer to a chromatography column equilibrated with the same buffer and comprising a non-ionic hydrophobic affinity material as the adsorbant. The column is washed once with the same buffer until a peak of material elutes, a second time with the same buffer with decreased ionic strength which lacks sodium chloride until a second peak elutes, and eluting the factor with unbuffered purified water. The eluate is desalted by membrane filtration and dried. The method is conducted in the absence of a volatile precipitating agent. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03072737-A3
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-03072737-A2 |
| priorityDate |
1986-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |