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filingDate 1986-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1987-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-8702065-A1
titleOfInvention Determination of identity between two organisms
abstract A method of determining whether two organisms are identical comprises subjecting a restriction endonuclease digest of genomic DNA of the first organism to electrophoresis; determining the positions of the DNA fragments thus separated which hybridise with one or more labelled DNA probes; and comparing these positions with the positions of DNA fragments binding to the or each probe and produced from genomic DNA of the second organism in like manner; an amount of probe DNA and one or more restriction endonucleases being used such that sufficient bands are revealed by the probing to achieve a sufficiently low probability (X) that the two organisms will have failed to have been distinguished when they appear identical from their band patterns, as determined by: X = Fq wherein F is a fraction representative of the proportion DNA fragments which are identical between restriction endonuclease digests of genomic DNA of pairs of independently-obtained organisms of the same species as the first and second organisms and q is the number of positions revealed by the probing.
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