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filingDate 1986-05-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1986-11-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber WO-8606839-A1
titleOfInvention A single vial technique for radiolabeling protein
abstract A method for radiolabeling protein such as antibody which is performed in a single reaction vessel. A sealed reaction vessel having a port for addition and withdrawal of reagents preferably by syringe is used. Reagents for coupling radioisotope to the protein are added to the vessel. For radioiodination procedures, vessels can be pre-coated with the iodine coupling agent iodogen. The protein and the radioisotope are then added to the vessel and the radiolabel reaction allowed to proceed. After the reaction is complete, a resin is added to the vessel to adsorb the uncoupled radioisotope. The entire reaction mixture is then withdrawn from the vessel and the resin is separated from the protein preferably by sterile filtration.
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