| abstract |
Improved peroxidase-containing and tetramethylbenzidine peroxidase-substrate reagents. The peroxidase-containing reagent consists of a buffered aqueous solutions comprising a peroxidase, such as horseradish peroxidase, or a peroxidase conjugate, and a specific substrate for the peroxidase, such as horseradish peroxidase, or a peroxidase conjugate, and a specific substrate for the peroxidase, such as tetramethylbenzidine, in the absence of peroxide. The peroxidase conjugate is suitably a conjugate of a peroxidase and a specific binding component of an assay. The tetramethylbenzidine peroxidase-substrate reagent comprises tetramethylbenzidine and BETA-cyclodextrin in aqueous solution. The solution may additionally contain a peroxide. One or both improved reagents may be included in an assay reagent kit. |