| abstract |
Methods for the detection and identification of unknown pathogens or genetic entities from samples suspected of containing the same involve the use of novel wicking techniques whereby sample DNAS/RNA becomes readily available on a porous, inert, positively charged support in single stranded form for hybridization with a hybridization probe having a nucleotide sequence substantiallly complementary to a nucleotide sequence of the sample DNA/RNA (Figs. 1-3). Such methods permit the detection and identification of unknown pathogens and genetic entities in a shorter period of time than that required by conventional methods. Novel lysis solutions for rapidly lysing bacterial cells without the aid of enzymes are also disclosed. |