| abstract |
"Two-site" or "sandwich" immunometric assay techniques for determination of the presence and/or concentration of antigenic substances in fluids using a combination of monoclonal and polyclonal antibodies. One antibody selected from a group consisting of monoclonalx and polyclonal(1-x) antibodies is presented in a soluble labeled form; a second antibody selected from a group consisting of monoclonal(1-x) and polyclonalx is presented bound to a solid carrier; and x is 0 or 1. The present invention further encompasses a kit comprising in association: (a) a soluble first antibody to an antigenic substance present in a fluid to be assayed, the first antibody being labeled and being selected from a group consisting of monoclonalx and polyclonal(1-x) antibodies; and (b) a second antibody to the antigenic substance, the second antibody being bound to a solid carrier and being selected from a group consisting of monoclonal(1-x) and polyclonalx antibodies; wherein x is 0 or 1. |