| abstract |
Cloning vectors with indicator genes, such as beta-galactosidase, which have been inactivated by frameshift. If a gene fragment with an open reading frame is inserted into a vector, the indicator gene may be reactivated. DNA from uncharacterized organisms can be fragmented, and protein-coding fragments can be easily identified by using the vectors. Polypeptides which can be produced by the identified fragments have a variety of useful purposes, including vaccines. This method allows for the simple production of a variety of valuable products, including polynucleotides, cells that produce useful polypeptides, vaccination agents and other polypeptides, antibodies, and assay kits. |