http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-8200253-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_971cf0f2a122f3ed17b2cb7d5cb9e3f3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_90bd484fddabf0df968c1603eacfdb41 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3a10266285c74bfe357b7b6dbca20cd0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f68254c2256a29224bb518f200d45ce9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0e1d075609f5f0c1f61211624d6c18c6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5cd764beda49ce980ba097614dbcc733 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5cf91409f51749cf781c13c88cc77419 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P13-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-087 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P13-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-80 |
| filingDate | 1981-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e4f268d3904e67ff42310f0e1861c6ca http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1efcc0f44b66a1dadf2eb5829aa13cee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3676e7b7cfeeb59762c706c7fef2d6de http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1432edebcde603ebe4776475a1ceae1e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_757bfb62ef28dd4070670d1161a34f62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7a44fc37653003ff25df9b1f47cd9e26 |
| publicationDate | 1982-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-8200253-A1 |
| titleOfInvention | Immobilized aminoacylase enzyme preparations,a process for the preparation thereof,and a process for the isolation of aminoacylase enzyme from the kidneys of mammals |
| abstract | Improved process for the isolation of amino acylase from the kidneys of mammals. According to the invention an aqueous extract of kidneys, prepared according to a known process, is subjected to a heat treatment at a temperature ranging from 60 to 80 C for 5 to 15 minutes, the mixture treated by heat is centrifugal, 200 to 300 g / liters of a salt corresponding to the general formula KxA (in which K represents a monovalent cation, A represents the anion of a mineral acid and x is equal to the valence of the anion) are added to the supernatant, the suspension obtained is centrifuged, the separate precipitate is dissolved in water, the solution is dialyzed against water and, optionally, the dialyzed solution or a solution of the enzyme, separated from the dialysate, in a buffer with a pH ranging from 6.5 to 8.5 is subjected to anion exchange chromatography, and finally, optionally, the active fractions obtained in the chromatographic step or a solution of the enzyme, precipitated from the active fractions by means of a salt corresponding to the general formula KxA, in a buffer of one pH values from 6.0 to 8.0 are subjected to gel chromatography on a filter in which the upper limit of the molecular weight of fractionation is higher than 70000, and the enzyme is precipitated from the active fractions by means of a salt corresponding to the general formula KxA already described. The invention also relates to an immobilized amino acylase consisting of a partially hydrolyzed copolymer of an acrylamide-M, M'-methylene-bis (acrylamide) of the Acrylex P type and of an amino acylase linked to this. copolymer by covalent bonds. This preparation of immobilized enzyme is prepared according to the invention in such a way that a partially hydrolyzed copolymer of the Acrylex P type is treated with a carbodiimide compound which is soluble in water or in an organic solvent at a temperature below 0 C, a solution of amino acylase having a pH ranging from 6.5 to 8.5 is applied to the resulting active support, the product |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9741214-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0083582-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0083582-A4 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-6235516-B1 |
| priorityDate | 1980-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 55.