http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-8000260-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_46443201668cc985a4ce2fb287770f99 |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2326-92 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-904 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-61 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-61 |
filingDate | 1979-05-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_988e277413de4053976ff78b794f2df8 |
publicationDate | 1980-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | WO-8000260-A1 |
titleOfInvention | Determination of triglycerides and enzyme reagents |
abstract | A method of determining triglycerides in biological fluids according to an enzymatic reaction. In the reaction, a biological fluid is added to an enzyme reagent comprising a lipase, glycerol dehydrogenase (GDH), pyridine nucleotide (PN), a buffer, a tetrazolium compound and diaphorase, whereby the triglycerides are enzymatically hydrolyzed, and the resulting glycerol is oxidized to form a reduced pyridine nucleotide (PN) which reacts with said tetrazolium compound in the presence of diaphorase to produce a colored formazan which is the reduced form of the tetrazolium, which color is measured at a wave length ranging from about 475 to about 525 nm. The lipase is an enzyme obtained from Chromobacterium viscosum. In the enzyme reagent, there is from about 5.0 to about 15.01. U./ml of the glycerol dehydrogenase (GDH) present. |
isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/FR-2520380-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/GB-2261729-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/FR-2449726-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-0045032-A1 |
priorityDate | 1978-07-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 561.