| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6419819278c4e69622dff8f635023155 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-04 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B30-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-02 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-04 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B30-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate |
2022-06-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c67a65bf34f1be6add49aa270691f164
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec45b6a476837a485b43be1fce732409
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5191a090029bb29dc2f8fa2f2bc3f351
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_256fe9daa6ddfd11bf6ecf7d848343e4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79b348a8c624b78ef87a3734f9408e04 |
| publicationDate |
2022-12-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2022270396-A1 |
| titleOfInvention |
Double-screening method |
| abstract |
[Problem] The purpose of the present invention is to provide a screening method equipped with a step which makes it possible to determine whether or not expression in a cell by a clone candidate, which is obtained by screening from a large-scale library before moving to a cell-based expression system, will be possible. [Solution] To provide a target molecule screening method equipped with: (a) a selection/screening step for constructing a library by selecting the DNA of a target molecule by using a non-cellular translation system, selecting the DNA of the target molecule from among the DNA fragments included in the library, and preparing a primary library by concentrating and screening the same; and (b) an expression/selection step for selecting a DNA fragment which codes a protein which can be produced in a living organism from among the proteins which can be produced from the target molecule included in the primary library obtained in the selection/screening step, and causing expression thereof using cells. |
| priorityDate |
2021-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |