| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ad6620a07bc98491a783de568d53ee13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4293d5e759e7a8c4422b3233f8a902ef
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fa5be6fa910cc16cabd1ca7801a4246a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_51b421699cb34dfd0302f78eb00b6f13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_12cf69f64dd1d1e9b59226707e92facc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_064e310b67ce2ae52d43cfd388f488ab |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate |
2022-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fddae9c377b23f499af914d9b4db4bfa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ffef20b3b6a335573e2a0ec200bac84d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_87599ad7b5c7fd0df0d36eaee0c60244
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_75a9b7e648b8001155624ec167b46426
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c4d61975df4ff804bf65aaf71f83191
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_795e272078811a37ed19a308a8a94ff8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5f14ae2bd6914245ba650dfd37484717
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04e62773c873fe17ec90085612917f37
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9312d303c96fe8c9988cb6d7091ca167
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0736e6fb201879daa82ea94e1b65a56c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c9566ea1ab4f22e31193143d52172fbe
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_685472acbcb192403323dd236e79fda0 |
| publicationDate |
2022-12-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2022266470-A1 |
| titleOfInvention |
Compositions and methods for pairwise sequencing |
| abstract |
The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer. |
| priorityDate |
2021-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |