http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022258437-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_35dbc60da9891d57c4047fb7b8689516 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-726 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6845 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2500-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2560-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6872 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2022-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_72a5c9c9654ad608da099f4eaa5471e0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e1bbc30d2e108c0b1aa2c3a58a35c4d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_048363c5fb16a85dbe4a17c7aa43473c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b46d8375007a8a9fb30ba51d98f8cc89 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c65f0cd5a3caf638067c5a146138fc72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_38492bac840be55e1d2b4be8aeb83b10 |
| publicationDate | 2022-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2022258437-A1 |
| titleOfInvention | Quantification of protein-protein interaction of membrane proteins using high-mass mass spectrometry |
| abstract | G-protein-coupled receptors (GPCRs) are important pharmaceutical targets for the treatment of a broad spectrum of diseases. Although there are structures of GPCRs in their active conformation with bound ligands and G-proteins, the detailed molecular interplay between the receptors and their signaling partners remains challenging to decipher. To address this, a high-throughput quantification method to quantify protein-protein interaction of membrane proteins is disclosed, comprising the steps of: a) providing a solution comprising a membrane protein, such as a GPCR, in the absence or presence of a chemical compound / compounds or ligands that can bind to or modulate the membrane protein;; b) adding a partner protein to the solution and after a predetermined time interval adding additionally a crosslinker that reacts with proteins' surface amino acid, such as lysines or other amino acids depending on the specific crosslinker that is used, in order to form chemical linkages that stabilize compound complexes of biomolecules; c) detecting and quantifying stabilized native and transient complexes of the biomolecules and the non-interacting biomolecule counterparts by mass spectrometry using a reference peak, i.e. in terms of a normalization strategy, to investigate the binding ability of the partner protein to the membrane protein. Thus, this high-sensitivity, high-throughput mass spectrometry method interrogates the first stage of signal transduction. The membrane protein and partner protein complex formation is detected as a proxy for the effect of ligands on membrane protein conformation and on coupling selectivity. The method requires only very little probe amounts, |
| priorityDate | 2021-06-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 332.