http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022247873-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_892c33e4735b1781cb423ea035722efc http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_76687a05be077bbf72841db9dc38f73b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2022-05-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56f6987545816542da97cfc04a5e5ae9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1b5706a055a5fc7135a51d1dbb68d06b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_02c0a1d332f43bdce3f400bff3576588 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_33d3308a80bbf7bd1319ecd911cfc1c3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3dfaa814eb0b9164adbb4b9de6b7f386 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6c082e83cbe36d2dfb9c0273e3f60a56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41009408b290cf104b2ff1bf52430907 |
| publicationDate | 2022-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2022247873-A1 |
| titleOfInvention | Engineered cas12i nuclease, effector protein and use thereof |
| abstract | Provided is an engineered Cas12i nuclease, which comprises one or more of the following mutations based on a reference Cas12i nuclease: (1) replacing one or more amino acids interacting with PAM in the reference Cas12i nuclease with positively charged amino acids; and/or (2) replacing one or more amino acids, involved in opening the double strands of DNA, in the reference Cas12i nuclease with amino acids with aromatic rings; and/or (3) replacing one or more amino acids, which interact with a single-stranded DNA substrate and are located in an RuvC domain in the reference Cas12i nuclease, with positively charged amino acids; and/or (4) replacing one or more amino acids interacting with a DNA-RNA double helix in the reference Cas12i nuclease with positively charged amino acids; and/or (5) replacing one or more polar or positively charged amino acids interacting with the DNA-RNA double helix in the reference Cas12i nuclease with hydrophobic amino acids. The engineered Cas12i nuclease has significantly improved gene editing efficiency and/or significantly reduced off-target phenomenon compared with the reference Cas12i nuclease. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023104185-A1 |
| priorityDate | 2021-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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