http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022247629-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2647663b7b285277bd2af8dc94bd96ef |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-20022 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1131 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2022-05-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ec38ad63f7e6fa2b21bd1d2ec65006a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d436c824fc92dd43a1180fd00e6a065 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ac09e367c6d57630b0c307c864db884 |
| publicationDate | 2022-12-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2022247629-A1 |
| titleOfInvention | Application of non-homologous double-stranded oligonucleotide fragment in gene knockout system |
| abstract | An application of a non-homologous double-stranded oligonucleotide fragment in a gene knockout system. Target gene knockout efficiency based on existing gene knockout systems is not high, and off-target sites are difficult to accurately position. In the method, a non-homologous double-stranded oligonucleotide fragment is combined for the first time with a gene knockout system for gene knockout, the non-homologous double-stranded oligonucleotide fragment can stimulate non-homologous terminal joining repair in cells and introduce more insertions or deletions; meanwhile, the non-homologous double-stranded oligonucleotide fragment can be inserted into a cleavage breakpoint, which can be used as a marker to monitor off-target sites, so as to accurately monitor off-target effects while helping to enhance the knockout efficiency of target genes. |
| priorityDate | 2021-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.