| abstract |
A method for separating active ingredients from Siraitia grosvenorii flowers, comprising: S1, extracting Siraitia grosvenorii flowers with water vapor to obtain wet flowers and a first extracting solution; S2, extracting the wet flowers with ethanol as a solvent at 25-65°C to obtain a second extracting solution and a first filter residue, concentrating the second extracting solution, and performing solid-liquid separation to obtain a third extracting solution and a wet residue; S3, concentrating the third extracting solution, and standing to obtain a crystal and a crystallization mother liquor; and S4, eluting a macroporous adsorption resin chromatographic column on the crystallization mother liquor sequentially with an acid solution, an alkali solution, and an alcohol solution to respectively obtain an acid eluent, an alkali eluent, and an alcohol eluent. According to the method, essential oil, flower dew, polysaccharide, Grosvenorine, alkaloid and triterpenoid saponin of Siraitia grosvenorii flowers can be obtained at the same time, and efficient comprehensive separation of Siraitia grosvenorii flowers is achieved. |