http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022243437-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0de2fbe5faca1f3e33ff1cd880df2873 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_829870c3d1719dc25f6939097d79a0e3 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 |
| filingDate | 2022-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_432e929c844855077b6aaf05bef68c35 |
| publicationDate | 2022-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | WO-2022243437-A1 |
| titleOfInvention | Sample preparation with oppositely oriented guide polynucleotides |
| abstract | The invention relates to a method for preparing a sample (400) comprising sample polynucleotides (602) for sequencing, wherein at least one of the sample polynucleotides comprises a known sequence (605), the method comprising: protecting (102) the ends of the sample polynucleotides, contacting (104) at least first and second nucleoprotein particles (608, 702, 704) with the protected sample polynucleotides, wherein each first nucleoprotein particle comprises an effector protein and a first guide polynucleotide (706) and each second nucleoprotein particle comprises an effector protein and a second guide polynucleotide (708), wherein the sequences of the first and second guide polynucleotides are different and are selected such that o the first guide polynucleotides cause the effector proteins to cut the known sequence at a first cleavage site (722); o the second guide polynucleotides cause the effector proteins to cut the known sequence at a second cleavage site (724); o wherein the first and second cleavage sites define an in-between sequence (614) as the part of the known sequence between the first and second cleavage sites (722, 724); o whereby the first and second guide polynucleotides respectively comprise a binding sequence (715, 713) whose position and orientation within the known sequence is selected such that when the sample is contacted with a sequencing adapter (616), the sequencing adapter selectively binds to the ends of the sample polynucleotide fragments created by the cutting which do not comprise the in- between sequence; adding (106) sequencing adapters to the sample. |
| priorityDate | 2021-05-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 112.